diff --git a/api/v1/clusterobjectset_types.go b/api/v1/clusterobjectset_types.go
index 0b0a93f3d0..4b2c8e0838 100644
--- a/api/v1/clusterobjectset_types.go
+++ b/api/v1/clusterobjectset_types.go
@@ -131,6 +131,10 @@ type ClusterObjectSetSpec struct {
 	//
 	// The maximum number of probes is 20.
 	//
+	// Once set, even if empty, the progressionProbes field is immutable, as it is
+	// part of the immutable content of a revision.
+	//
+	// +kubebuilder:validation:XValidation:rule="self == oldSelf || oldSelf.size() == 0", message="progressionProbes is immutable"
 	// +kubebuilder:validation:MinItems=1
 	// +kubebuilder:validation:MaxItems=20
 	// +listType=atomic
diff --git a/api/v1/clusterobjectset_types_test.go b/api/v1/clusterobjectset_types_test.go
index f59251150e..dbb3db6743 100644
--- a/api/v1/clusterobjectset_types_test.go
+++ b/api/v1/clusterobjectset_types_test.go
@@ -140,6 +140,31 @@ func TestClusterObjectSetImmutability(t *testing.T) {
 				cos.Spec.CollisionProtection = CollisionProtectionNone
 			},
 		},
+		"progressionProbes may be initially unset": {
+			spec: ClusterObjectSetSpec{
+				Group:               "test-group",
+				LifecycleState:      ClusterObjectSetLifecycleStateActive,
+				Revision:            1,
+				CollisionProtection: CollisionProtectionPrevent,
+			},
+			updateFunc: func(cos *ClusterObjectSet) {
+				cos.Spec.ProgressionProbes = []ProgressionProbe{validProbe()}
+			},
+			allowed: true,
+		},
+		"progressionProbes are immutable once set": {
+			spec: ClusterObjectSetSpec{
+				Group:               "test-group",
+				LifecycleState:      ClusterObjectSetLifecycleStateActive,
+				Revision:            1,
+				CollisionProtection: CollisionProtectionPrevent,
+				ProgressionProbes:   []ProgressionProbe{validProbe()},
+			},
+			updateFunc: func(cos *ClusterObjectSet) {
+				cos.Spec.ProgressionProbes = []ProgressionProbe{validProbe(), validProbe()}
+			},
+			expectedError: "progressionProbes is immutable",
+		},
 		"lifecycleState cannot be un-archived": {
 			spec: ClusterObjectSetSpec{
 				Group:               "test-group",
diff --git a/applyconfigurations/api/v1/clusterobjectsetspec.go b/applyconfigurations/api/v1/clusterobjectsetspec.go
index 241a21b425..1698ab7208 100644
--- a/applyconfigurations/api/v1/clusterobjectsetspec.go
+++ b/applyconfigurations/api/v1/clusterobjectsetspec.go
@@ -88,6 +88,9 @@ type ClusterObjectSetSpecApplyConfiguration struct {
 	//
 	// The maximum number of probes is 20.
 	//
+	// Once set, even if empty, the progressionProbes field is immutable, as it is
+	// part of the immutable content of a revision.
+	//
 	// <opcon:experimental>
 	ProgressionProbes []ProgressionProbeApplyConfiguration `json:"progressionProbes,omitempty"`
 	// collisionProtection specifies the default collision protection strategy for all objects
diff --git a/helm/olmv1/base/object-controller/crd/experimental/olm.operatorframework.io_clusterobjectsets.yaml b/helm/olmv1/base/object-controller/crd/experimental/olm.operatorframework.io_clusterobjectsets.yaml
index 67e2d8e2d0..5d83203f1b 100644
--- a/helm/olmv1/base/object-controller/crd/experimental/olm.operatorframework.io_clusterobjectsets.yaml
+++ b/helm/olmv1/base/object-controller/crd/experimental/olm.operatorframework.io_clusterobjectsets.yaml
@@ -307,6 +307,9 @@ spec:
                   a ConfigMap if that particular object does not pass the probe check.
 
                   The maximum number of probes is 20.
+
+                  Once set, even if empty, the progressionProbes field is immutable, as it is
+                  part of the immutable content of a revision.
                 items:
                   description: ProgressionProbe provides a custom probe definition,
                     consisting of an object selection method and assertions.
@@ -553,6 +556,9 @@ spec:
                 minItems: 1
                 type: array
                 x-kubernetes-list-type: atomic
+                x-kubernetes-validations:
+                - message: progressionProbes is immutable
+                  rule: self == oldSelf || oldSelf.size() == 0
               revision:
                 description: |-
                   revision is a required, immutable sequence number identifying a specific
diff --git a/manifests/experimental-e2e.yaml b/manifests/experimental-e2e.yaml
index 355dc36eef..38c406056c 100644
--- a/manifests/experimental-e2e.yaml
+++ b/manifests/experimental-e2e.yaml
@@ -2279,6 +2279,9 @@ spec:
                   a ConfigMap if that particular object does not pass the probe check.
 
                   The maximum number of probes is 20.
+
+                  Once set, even if empty, the progressionProbes field is immutable, as it is
+                  part of the immutable content of a revision.
                 items:
                   description: ProgressionProbe provides a custom probe definition,
                     consisting of an object selection method and assertions.
@@ -2525,6 +2528,9 @@ spec:
                 minItems: 1
                 type: array
                 x-kubernetes-list-type: atomic
+                x-kubernetes-validations:
+                - message: progressionProbes is immutable
+                  rule: self == oldSelf || oldSelf.size() == 0
               revision:
                 description: |-
                   revision is a required, immutable sequence number identifying a specific
diff --git a/manifests/experimental.yaml b/manifests/experimental.yaml
index 29028f1ec7..f08813c1a6 100644
--- a/manifests/experimental.yaml
+++ b/manifests/experimental.yaml
@@ -2240,6 +2240,9 @@ spec:
                   a ConfigMap if that particular object does not pass the probe check.
 
                   The maximum number of probes is 20.
+
+                  Once set, even if empty, the progressionProbes field is immutable, as it is
+                  part of the immutable content of a revision.
                 items:
                   description: ProgressionProbe provides a custom probe definition,
                     consisting of an object selection method and assertions.
@@ -2486,6 +2489,9 @@ spec:
                 minItems: 1
                 type: array
                 x-kubernetes-list-type: atomic
+                x-kubernetes-validations:
+                - message: progressionProbes is immutable
+                  rule: self == oldSelf || oldSelf.size() == 0
               revision:
                 description: |-
                   revision is a required, immutable sequence number identifying a specific